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Plant CellMtCEP1ڵ͵ޣ֮ķƽ

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A CEP Peptide Receptor-like Kinase Regulates Auxin Biosynthesis and Ethylene Signaling to Coordinate Root Growth and Symbiotic Nodulation in Medicago truncatula

һFugui Zhu

һλйũҵѧ

ͨѶTao Wang


 Abstract 


عBecause of the high energy consumed during symbiotic nitrogen fixation, legumes must balance growth and symbiotic nodulation.


Both lateral roots and nodules form on the root system and the developmental coordination of these organs according to reduced nitrogen (N) availability remains elusive.


ҪWe show that the Compact Root Architecture 2 (MtCRA2) receptor-like kinase is essential to promote the initiation of early symbiotic nodulation and to inhibit root growth in response to low-N.


غϳMtCEP1 peptides can activate MtCRA2 under N-starvation conditions, leading to a repression of MtYUC2auxin biosynthesis gene expression, and therefore of auxin root responses. Accordingly, the compact root architecture phenotype of cra2 can be mimicked by an auxin treatment or by over-expressing MtYUC2, and conversely, a treatment with YUC inhibitors or a MtYUC2 knock-out rescues the cra2 root phenotype.


ϩźThe MtCEP1-activated CRA2 can additionally interact with and phosphorylate the MtEIN2 ethylene signaling component at Ser643 and Ser924, preventing its cleavage and therefore repressing ethylene responses, thus locally promoting the root susceptibility to rhizobia. In agreement, the cra2 low nodulation phenotype is rescued by an ein2 mutation.


Overall, by reducing auxin biosynthesis and inhibiting ethylene signaling, the MtCEP1/MtCRA2 pathway balances root and nodule development under low-N conditions.

Model for the action of MtCRA2 in coordinating root architecture and symbiotic nodulation under low-N conditions.


 ժ  Ҫ 


Ϊ̵ҪĴ˶ֲƽ빲֮Ĺϵ͸ڸϵͳγɣȻЩ֮ڿõԴٺķЭУ߷޼ޣ弤øMtCRA2Ӧڵ͵ٽڹʼƸMtCEP1Ŀڵ¼MtCRA2غϳɻMtYUC2ıƣӶƸӦͨش߹MtYUC2ģcra2ͻյĸṹͣ෴ͨYUCƼMtYUC2óָܹcra2ͻı͡⣬MtCEP1CRA2Żϩźͨ·MtEIN2Ser643Ser924ữõף䲻иӶϩӦ˾ֲٽڸԡcra2ͻȽٵıҲܹͨein2ͻָϣ޼ޣڵ͵£MtCEP1/MtCRA2ͨ·ͨغϳɺϩźתƽ֮ƽ⡣


p.s. ϩźEIN2λڰʵĤϣڴϩʱC˱ˣεϩźš


 ͨѶ 


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˼飺

1986꣬ɽũҵѧѧʿ

1989꣬йũҵѧ˶ʿ

1998꣬йũҵѧʿ


оƹܻоͷ֣

1. ޣӻо

2. ޣ-̵ػо

3. ּºƷ


doi: 10.1105/tpc.19.00428


Journal: Plant Cell

Published date: June 25, 2020



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